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人抗乙型肝炎病毒核心IgM抗體酶免試劑盒,(HBcAb-IgM)ELISA檢測試劑盒 ,英文名:HBcAb-IgM ELISA Kit ,英文縮寫:HBcAb-IgM ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗乙型肝炎病毒核心IgM抗體ELISA檢測試劑盒,HBcAb-IgM 檢測試劑盒, HBcAb-IgM 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗乙型肝炎病毒核心IgM抗體酶免試劑盒,(HBcAb-IgM)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重
周期素依賴性激酶4(CDK4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase 4 (CDK4)48T/96TY09135Homo sapiens (Human)
殺菌性/通透性增加蛋白(BPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bactericidal/Permeability Increasing Protein (BPI)48T/96TY09136Homo sapiens (Human)
毒蕈堿型膽堿受體M5(CHRM5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Muscarinic 5 (CHRM5)48T/96TY09137Homo sapiens (Human)
纖溶酶原(Plg)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen (Plg)48T/96TY09138Homo sapiens (Human)
纖維蛋白肽A(FPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinopeptide A (FPA)48T/96TY09139Homo sapiens (Human)
酰化刺激蛋白(ASP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acylation Stimulating Protein (ASP)48T/96TY09140Homo sapiens (Human)
角蛋白19(KRT19)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Keratin 19 (KRT19)48T/96TY09141Homo sapiens (Human)
角蛋白20(KRT20)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Keratin 20 (KRT20)48T/96TY09142Homo sapiens (Human)
Kruppel樣因子4(KLF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kruppel Like Factor 4, Gut (KLF4)48T/96TY09143Homo sapiens (Human)
ATP結(jié)合盒轉(zhuǎn)運蛋白A1(ABCA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATP Binding Cassette Transporter A1 (ABCA1)48T/96TY09144Homo sapiens (Human)
利鉀尿肽(KP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kaliuretic Peptide (KP)48T/96TY09145Homo sapiens (Human)
心型脂肪酸結(jié)合蛋白(FABP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fatty Acid Binding Protein 3, Muscle And Heart (FABP3)48T/96TY09146Homo sapiens (Human)
TGFβ誘導(dǎo)早期應(yīng)答基因1(TIEG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for TGF Beta Inducible Early Response Gene 1 (TIEG1)48T/96TY09147Homo sapiens (Human)
胸腺表達(dá)趨化因子(TECK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thymus Expressed Chemokine (TECK)48T/96TY09148Homo sapiens (Human)
雄激素受體(AR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Androgen Receptor (AR)48T/96TY09149Homo sapiens (Human)
TGFβ誘導(dǎo)早期應(yīng)答基因1(TIEG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for TGF Beta Inducible Early Response Gene 1 (TIEG1)48T/96TY09150Mus musculus (Mouse)
血管抑素(ANG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiostatin (ANG)48T/96TY09151Homo sapiens (Human)
延伸蛋白A(ELOA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Elongin A (ELOA)48T/96TY09152Homo sapiens (Human)
基質(zhì)金屬蛋白酶26(MMP26)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 26 (MMP26)48T/96TY09153Homo sapiens (Human)
26S-蛋白酶體(26S-PSM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 26S-Proteasome (26S-PSM)48T/96TY09154Homo sapiens (Human)
谷氨酸脫羧酶2(GAD2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Decarboxylase 2, Acid (GAD2)48T/96TY09155Homo sapiens (Human)
Smad同源物7(Smad7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mothers Against Decapentaplegic Homolog 7 (Smad7)48T/96TY09156Mus musculus (Mouse)
接觸蛋白3(CNTN3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Contactin 3 (CNTN3)48T/96TY09157Homo sapiens (Human)
胰島素自身抗體(IAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Autoantibody (IAA)48T/96TY09158Homo sapiens (Human)
胰多肽(PP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pancreatic Polypeptide (PP)48T/96TY09159Homo sapiens (Human)
胰高血糖素(GC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucagon (GC)48T/96TY09160Homo sapiens (Human)
激肽原1(KNG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kininogen 1 (KNG1)48T/96TY09161Homo sapiens (Human)
胱天蛋白酶13(CASP13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caspase 13 (CASP13)48T/96TY09162Bos taurus; Bovine (Cattle)
孕酮受體(PGR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Progesterone Receptor (PGR)48T/96TY09163Homo sapiens (Human)
早老素2(PS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Presenilin 2 (PS2)48T/96TY09164Homo sapiens (Human)
膜輔蛋白(MCP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Membrane Cofactor Protein (MCP)48T/96TY09165Homo sapiens (Human)
激素敏感性脂肪酶(LIPE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipase, Hormone Sensitive (LIPE)48T/96TY09166Homo sapiens (Human)
腦型脂肪酸結(jié)合蛋白(FABP7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fatty Acid Binding Protein 7, Brain (FABP7)48T/96TY09167Homo sapiens (Human)
組織多肽特異性抗原(TPS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Polypeptide Specific Antigen (TPS)48T/96TY09168Homo sapiens (Human)
Smad同源物7(Smad7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mothers Against Decapentaplegic Homolog 7 (Smad7)48T/96TY09169Rattus norvegicus (Rat)
Smad同源物7(Smad7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mothers Against Decapentaplegic Homolog 7 (Smad7)48T/96TY09170Homo sapiens (Human)
Smad同源物1(Smad1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mothers Against Decapentaplegic Homolog 1 (Smad1)48T/96TY09171Mus musculus (Mouse)
Smad同源物1(Smad1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mothers Against Decapentaplegic Homolog 1 (Smad1)48T/96TY09172Rattus norvegicus (Rat)
Smad同源物1(Smad1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mothers Against Decapentaplegic Homolog 1 (Smad1)48T/96TY09173Homo sapiens (Human)
含血管性血友病因子A域蛋白3A(vWA3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Von Willebrand Factor A Domain Containing Protein 3A (vWA3A)48T/96TY09174Homo sapiens (Human)
Ⅰ型膠原交聯(lián)氨基端肽(NTXⅠ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cross Linked N-opeptide Of Type I Collagen (NTXI)48T/96TY09175Homo sapiens (Human)
分泌型免疫球蛋白A(sIgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretory Immunoglobulin A (sIgA)48T/96TY09176Homo sapiens (Human)
果糖二磷酸醛縮酶A(ALDOA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aldolase A, Fructose Bisphosphate (ALDOA)48T/96TY09177Homo sapiens (Human)
27kDa熱休克蛋白(HSPB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 27kDa Protein 1 (HSPB1)48T/96TY09178Homo sapiens (Human)
27kDa熱休克蛋白(HSPB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 27kDa Protein 1 (HSPB1)48T/96TY09179Rattus norvegicus (Rat)
27kDa熱休克蛋白(HSPB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 27kDa Protein 1 (HSPB1)48T/96TY09180Mus musculus (Mouse)
Ⅰ型膠原交聯(lián)氨基端肽(NTXⅠ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cross Linked N-opeptide Of Type I Collagen (NTXI)48T/96TY09181Mus musculus (Mouse)
Ⅰ型膠原交聯(lián)氨基端肽(NTXⅠ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cross Linked N-opeptide Of Type I Collagen (NTXI)48T/96TY09182Rattus norvegicus (Rat)
50kDa核孔蛋白(NUP50)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoporin 50kDa (NUP50)48T/96T Y09183Homo sapiens (Human)
CCAAT增強子結(jié)合蛋白α(CEBPα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for CCAAT/Enhancer Binding Protein Alpha (CEBPa)48T/96TY09184Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。