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小鼠血小板因子3酶免試劑盒,(PF-3)ELISA檢測試劑盒 ,英文名:PF-3 ELISA Kit ,英文縮寫:PF-3 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠血小板因子3ELISA檢測試劑盒,PF-3 檢測試劑盒, PF-3 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠血小板因子3酶免試劑盒,(PF-3)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
苯乙肼裂解酶(NRD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nardilysin (NRD1)48T/96TY15585Homo sapiens (Human)
甘氨酸脒基轉(zhuǎn)移酶(GATM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycine Amidinotransferase (GATM)48T/96TY15586Homo sapiens (Human)
精氨酸脫羧酶(ADC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arginine Decarboxylase (ADC)48T/96TY15587Homo sapiens (Human)
腺苷高半胱氨酸水解酶(AHCY)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenosylhomocysteinase (AHCY)48T/96TY15588Homo sapiens (Human)
甘氨酸脫氫酶(GLDC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycine Dehydrogenase (GLDC)48T/96TY15589Homo sapiens (Human)
組氨酸脫羧酶(HDC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Histidine Decarboxylase (HDC)48T/96TY15590Homo sapiens (Human)
磷脂酰絲氨酸脫羧酶(PISD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphatidylserine Decarboxylase (PISD)48T/96TY15591Homo sapiens (Human)
磷酸泛酰半胱氨酸脫羧酶(PPCDC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphopantothenoylcysteine Decarboxylase (PPCDC)48T/96TY15592Homo sapiens (Human)
尿卟啉原脫羧酶(UROD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Uroporphyrinogen Decarboxylase (UROD)48T/96TY15593Homo sapiens (Human)
毛透明蛋白(TCHH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Trichohyalin (TCHH)48T/96TY15594Homo sapiens (Human)
脂質(zhì)運載蛋白12(LCN12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipocalin 12 (LCN12)48T/96TY15595Homo sapiens (Human)
亞精胺合酶(SRM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Spermidine Synthase (SRM)48T/96TY15596Homo sapiens (Human)
法尼基轉(zhuǎn)移酶α(FNTα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Farnesyltransferase Alpha (FNTa)48T/96TY15597Homo sapiens (Human)
類脂肪酶A(LPHA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipophilin A (LPHA)48T/96TY15598Homo sapiens (Human)
脂素1(LPIN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipin 1 (LPIN1)48T/96TY15599Homo sapiens (Human)
脂素2(LPIN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipin 2 (LPIN2)48T/96TY15600Homo sapiens (Human)
脂素3(LPIN3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipin 3 (LPIN3)48T/96TY15601Homo sapiens (Human)
粘脂蛋白1(MCOLN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucolipin 1 (MCOLN1)48T/96TY15602Homo sapiens (Human)
粘脂蛋白2(MCOLN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucolipin 2 (MCOLN2)48T/96TY15603Homo sapiens (Human)
粘脂蛋白3(MCOLN3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucolipin 3 (MCOLN3)48T/96TY15604Homo sapiens (Human)
Ly6/神經(jīng)毒素1(LYNX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ly6/Neurotoxin 1 (LYNX1)48T/96TY15605Homo sapiens (Human)
溶血卵磷脂?;D(zhuǎn)移酶1(LPCAT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysophosphatidylcholine Acyltransferase 1 (LPCAT1)48T/96TY15606Homo sapiens (Human)
溶血卵磷脂?;D(zhuǎn)移酶2(LPCAT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysophosphatidylcholine Acyltransferase 2 (LPCAT2)48T/96TY15607Homo sapiens (Human)
溶血卵磷脂酰基轉(zhuǎn)移酶3(LPCAT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysophosphatidylcholine Acyltransferase 3 (LPCAT3)48T/96TY15608Homo sapiens (Human)
溶血卵磷脂?;D(zhuǎn)移酶4(LPCAT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysophosphatidylcholine Acyltransferase 4 (LPCAT4)48T/96TY15609Homo sapiens (Human)
膽堿/乙醇胺磷酸轉(zhuǎn)移酶1(CEPT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Choline/Ethanolamine Phosphotransferase 1 (CEPT1)48T/96TY15610Homo sapiens (Human)
乙醇胺磷酸轉(zhuǎn)移酶1(EPT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ethanolamine Phosphotransferase 1 (EPT1)48T/96TY15611Homo sapiens (Human)
苯基乙醇胺-N-甲基轉(zhuǎn)移酶(PNMT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phenylethanolamine-N-Methyltransferase (PNMT)48T/96TY15612Homo sapiens (Human)
磷脂酰乙醇胺-N-甲基轉(zhuǎn)移酶(PEMT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphatidylethanolamine-N-Methyltransferase (PEMT)48T/96TY15613Homo sapiens (Human)
乙醇胺激酶1(ETNK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ethanolamine Kinase 1 (ETNK1)48T/96TY15614Homo sapiens (Human)
乙醇胺激酶2(ETNK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ethanolamine Kinase 2 (ETNK2)48T/96TY15615Homo sapiens (Human)
鈉/葡萄糖協(xié)同轉(zhuǎn)運蛋白3(SGLT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sodium/Glucose Cotransporter 3 (SGLT3)48T/96TY15616Homo sapiens (Human)
鈉/鉀/鈣交換因子1(NCKX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sodium/Potassium/Calcium Exchanger 1 (NCKX1)48T/96TY15617Homo sapiens (Human)
鈉/鉀/鈣交換因子2(NCKX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sodium/Potassium/Calcium Exchanger 2 (NCKX2)48T/96TY15618Homo sapiens (Human)
焦磷酸酶2(PPA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pyrophosphatase 2 (PPA2)48T/96TY15619Homo sapiens (Human)
焦磷酸酶1(PPA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pyrophosphatase 1 (PPA1)48T/96TY15620Homo sapiens (Human)
堿性神經(jīng)酰胺酶3(ACER3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alkaline Ceramidase 3 (ACER3)48T/96TY15621Homo sapiens (Human)
半乳糖神經(jīng)酰胺酶(GALC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Galactosylceramidase (GALC)48T/96TY15622Homo sapiens (Human)
耐扭蛋白3A(TOR3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Torsin 3A (TOR3A)48T/96TY15623Homo sapiens (Human)
耐扭蛋白1B(TOR1B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Torsin 1B (TOR1B)48T/96TY15624Homo sapiens (Human)
耐扭蛋白1A(TOR1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Torsin 1A (TOR1A)48T/96TY15625Homo sapiens (Human)
腦膜瘤基因1(MN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Meningioma 1 (MN1)48T/96TY15626Homo sapiens (Human)
腺苷酸激酶5(AK5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenylate Kinase 5 (AK5)48T/96TY15627Homo sapiens (Human)
糞卟啉原氧化酶(CPOX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coproporphyrinogen Oxidase (CPOX)48T/96TY15628Homo sapiens (Human)
Cereblon蛋白(CRBN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cereblon (CRBN)48T/96TY15629Homo sapiens (Human)
原肌球調(diào)節(jié)蛋白4(TMOD4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tropomodulin 4 (TMOD4)48T/96TY15630Homo sapiens (Human)
原肌球調(diào)節(jié)蛋白3(TMOD3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tropomodulin 3 (TMOD3)48T/96TY15631Homo sapiens (Human)
原肌球調(diào)節(jié)蛋白2(TMOD2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tropomodulin 2 (TMOD2)48T/96TY15632Homo sapiens (Human)
原肌球調(diào)節(jié)蛋白1(TMOD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tropomodulin 1 (TMOD1)48T/96TY15633Homo sapiens (Human)
遮蔽蛋白(OBSCN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Obscurin (OBSCN)48T/96TY15634Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。